Composite

Part:BBa_K208046:Design

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)

Lac Promoter/RBS/PelB/GFP(Hawaii08 Silver)/Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 941


Design Notes

Lac I promoter with RBS, PelB secretion tag, Hawaii's GFP and two terminators in pSB1AK3 vector.

Source

Composite

References